Anti-FUS (internal region) antibody produced in rabbit
SIGMA/SAB4200511 - affinity isolated antibody
Synonym: Anti-HNRNPP2; Anti-TLS; Anti-fus-like protein; Anti-fused in sarcoma, ALS6, FUS1, POMP75, 75 kDa DNA-pairing protein; Anti-fusion gene in myxoid liposarcoma; Anti-heterogeneous nuclear ribonucleoprotein P2; Anti-translocated in liposarcoma protein
Product Type: Chemical
Immunofluorescence HepG2 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-FUS (Cat. No. SAB4200511). The antibody was developed using Goat Anti-Rabbit IgG, Atto-488 conjugate (Cat. No. 18772).
Immunoblotting Whole extract of HEK-293T cells was separated on SDS-PAGE and probed with Rabbit Anti-FUS (Cat. No. SAB4200511). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + FUS immunizing peptide 3. 1 μg/mL antibody 4. 0.5 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using human HepG2 or MCF7 cells. |
| western blot: 0.5-1.0 μg/mL using whole extracts of human HEK-293T or G361 cells. | |
| UniProt accession no. | P35637  |
| Application: | Anti-FUS (internal region) antibody produced in rabbit may be used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Anti-FUS (internal region) recognizes human FUS. |
| Biochem/physiol Actions: | Fused in sarcoma (FUS), also called translocation in liposarcoma or Tumor lysis syndrome (TLS), plays a key role in RNA splicing, DNA repair and transcriptional regulation. It is a RNA/DNA binding protein implicated in various diseases. Mutations in the FUS gene is implicated in familial amyotrophic lateral sclerosis (FALS). Chromosomal translocation of FUS/TLS is found in human cancers and results in the production of oncogenic FUS fusion proteins. FUS has been implicated in a broadening spectrum of neurodegenerative disorders and is a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1) and SCA3. The majority of the FUS mutations have been identified in the C-terminal nuclear localization signal (NLS). TAR DNA-binding protein 43 (TDP-43) and FUS have been identified in amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FLTD) and familial amyotrophic lateral sclerosis (FALS). Pathological FUS inclusions are mostly found in the cytosol of neurons and glial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fused in sarcoma (FUS) is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complex. FUS gene is mapped to human chromosome 16p11.2. FUS is located predominantly in the nucleus. |
| Immunogen: | peptide corresponding to an internal region of human FUS, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 4 amino acids in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |