Anti-Arc antibody produced in rabbit
SIGMA/SAB4200515 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Arc antibody produced in Rabbit (Cat. No. SAB4200515) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Arc (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 0.5 μg/mL.
Immunohistochemistry Localization of ARC in rat brain hippocampus (dentate gyrus). Formalin-fixed paraffin-embedded tissue was stained with 20 μg/mL. Anti-ARC (Cat. No. SAB4200515) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×40 magnification).
Immunohistochemistry Localization of ARC in rat cerebellum. Formalin-fixed paraffin-embedded tissue was stained with 20 μg/mL. Anti-ARC (Cat. No. SAB4200515) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (x10 magnification).
Immunoblotting Cell lysates were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-ARC (Cat. No. SAB4200515) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. U937 3. U937. Antibody: 1.5 μg/mL + ARC immunizing peptide
Immunoblotting Cell lysates of HEK-293T overexpressing human ARC (lanes 1,3) and HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-ARC (Cat. No. SAB4200515) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody: 0.5 μg/mL 2. Antibody: 0.5 μg/mL 3. Antibody: 0.5 μg/mL + ARC immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~55 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded rat brain |
| western blot: 1.5-3 μg/mL using U937 cell extracts | |
| UniProt accession no. | Q7LC44  |
| Biochem/physiol Actions: | Activity regulated cytoskeleton associated protein (Arc) plays a vital role in memory formation. It also facilitates activity-dependent plasticity of dendrites. Mutation in the gene has been associated with the development of idiopathic generalized epilepsy (IGE). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Activity regulated cytoskeleton associated protein (Arc) is encoded by the gene mapped to human chromosome 8q24.3. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human Arc, conjugated to KLH. The corresponding sequence is identical in rat and mouse Arc. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |