Anti-USP8 antibody, Mouse monoclonal
SIGMA/SAB4200527 - clone US872, purified from hybridoma cell culture
Synonym: Anti-
Product Type: Chemical
Immunofluorescence A549 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 5 μg/mL Mouse Anti-USP8 Clone: US872 (Cat. No. SAB4200527). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting MCF7 cell extract was separated on SDS-PAGE and probed with Mouse Anti-USP8 Clone: US872 (Cat. No. SAB4200527). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 0.5 μg/mL antibody + USP8 immunizing peptide (human, 696-710)
Immunoblotting Cell extracts were separated on SDS-PAGE and probed with 1 μg/mL Mouse Anti-USP8 Clone: US872 (Cat. No. SAB4200527). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. MDCK 2. MDBK 3. COS-7 4. PC-12 5. 4T1 6. Hepa 1-6 7. HT-29 8. HeLa 9. MDA-MB-231
Flow Cytometry 1×106 MCF7 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with 5 μg Mouse Anti-USP8 Clone: US872 (Cat. No. SAB4200527) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody. Purple histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | US872, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~127 kDa |
| antigen ~70 kDa | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, bovine, monkey, mouse, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-10 μg/test using MCF7 cells |
| indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells | |
| western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts | |
| UniProt accession no. | P40818  |
| Application: | Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry. |
| Biochem/physiol Actions: | Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs). |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |