Anti-HMGCR antibody produced in rabbit
SIGMA/SAB4200528 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-
Product Type: Chemical
Immunofluorescence
Mevastatin-treated HepG2 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1 μg/mL Rabbit Anti-HMGCR (Cat. No. SAB4200528). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Mevastatin-treated HepG2 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1 μg/mL Rabbit Anti-HMGCR (Cat. No. SAB4200528). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of Mevastatin-treated HepG2 cells was separated on SDS-PAGE and probed with Rabbit Anti-HMGCR (Cat. No. SAB4200528). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1.5 μg/mL 2. 1.5 μg/mL untreated HepG2 cells 3. 1.5 μg/mL + HMGCR immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1.0-2.0 μg/mL using mevastatin-treated HepG2 cells. |
| western blot: 1.5-3.0 μg/mL using extracts of mevastatin-treated HepG2 cells. | |
| UniProt accession no. | P04035  |
| Application: | Anti-HMGCR antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | 3-hydroxy-3-methylglutary |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The 3-hydroxy-3-methylglutary |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human HMGCR, conjugated to KLH. The corresponding sequence is identical in human HMGCR isoform 2 and has 87% sequence identity in mouse HMGCR and 81% identity in rat HMGCR. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |