Anti-HMGCR (internal) antibody produced in rabbit

SIGMA/SAB4200529 - ~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-3-hydroxy-3-methylglutaryl-CoA reductase, LDLCQ3

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SAB4200529-200UL	200 µL	$565.00 1/EA	Add To Favorites

Western Blotting Whole extracts of Rat and Mouse liver were separated on SDS-PAGE and probed with 1.5 μg/mL Anti-HMGCR (internal) antibody produced in Rabbit (Cat. No. SAB4200529). The antibody was developed using 1:25,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. Rat liver 2. Mouse liver 3. Negative Control 4. Rat liver + inhibitor 5. Mouse liver + inhibitor

Properties
Descriptions
Safety Info.
Basic Data

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
concentration	~1.0 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
mol wt	antigen ~100 kDa
Quality Level	200
shipped in	dry ice
species reactivity	human, mouse, rat
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	western blot: 1.5-3.0 μg/mL using extracts of mevastatin-treated HepG2 cells, and of mouse and rat liver microsomes.
UniProt accession no.	P04035

Biochem/physiol Actions:	3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) plays a major role in mevalonate biosynthesis, which is a rate limiting step of cholesterol biosynthesis in the liver. Additionally, it also plays a key role in various biological process such as, embryogenesis and cancer. Elevated expression of the gene is associated with the development of gastric cancer(GC) and glioblastoma cells. Thus, HMGCR can be considered as a potent therapeutic target for GC and glioblastoma.
Biochem/physiol Actions:	The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) acts as the rate-limiting step in cholesterol synthesis. Polymorphism in HMGCR results in late-onset Alzheimer′s disease. It induces the growth and migration of the cancer cells.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) is a transmembrane glycoprotein, encoded by the gene mapped to human chromosome 5q13.3-q14. HMGCR has a molecular mass of 97kDa and is mainly localized to smooth endoplasmic reticulum. The encoded protein is predominantly expressed in liver tissues.
General description:	The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) codes for a part of the statin-binding domain of the enzyme. This gene is located on human chromosome 5q13. HMGCR has a molecular mass of 97kDa and is mainly localized to smooth endoplasmic reticulum. The encoded protein is predominantly expressed in liver tissues.
Immunogen:	synthetic peptide corresponding to an internal sequence of human HMGCR, conjugated to KLH. The corresponding sequence is identical in human HMGCR isoform 2 and highly conserved in mouse (89% identity) and in rat (83% identity) HMGCR.
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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