Anti-ZNF746 antibody produced in rabbit
SIGMA/SAB4200530 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-ZNF746 antibody produced in Rabbit (Cat. No. SAB4200530) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing ZNF746 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 0.1 μg/mL.
Immunofluorescence Cytoplasmic localization of ZNF746 in SH-SY-5Y cells. Fixed cells were stained with 5 μg/mL Anti-ZNF746 (Cat. No. SAB4200530) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell lysates and tissue extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-ZNF746 (Cat. No. SAB4200530) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. K562 2. HepG2 3. SH-SY-5Y 4. Rat brain (S2 fraction) 5. NIH3T3 6. NTERA-2 7. NTERA-2. Antibody: 1 μg/mL + ZNF746 immunizing peptide
Immunoblotting Cell lysates of HEK-293T overexpressing human ZNF746 (lanes 1,3) and HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-ZNF746 (Cat. No. SAB4200530) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody: 0.1 μg/mL 2. Antibody: 0.1 μg/mL 3. Antibody: 0.1 μg/mL + ZNF746 immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~90 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using SH-SY-5Y cells. |
| western blot: 1.0-2.0 μg/mL using using rat brain extracts (S2 fraction) and NIH3T3 fibroblast cell extracts. | |
| UniProt accession no. | Q6NUN9  |
| Application: | Anti-ZNF746 antibody produced in rabbit has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) accumulates in Parkinson′s disease (PD) models and in human PD brain. Hence it play a role in the progress of PD disease. ZNF746 is also regulated by the E3 ubiquitin ligase, parkin. In addition, overexpression of ZNF746 leads to the selective loss of dopaminergic neurons in the substantia nigra. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) is a 644 amino acid protein. It has a Kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C terminals. This protein is highly conserved among human, mouse and rat, and widely expressed in many tissues. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human ZNF746, conjugated to KLH. The corresponding sequence is identical in human ZNF746 isoform 2, and in mouse and rat ZNF746. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |