Anti-Claudin-1 (C-terminal) antibody produced in rabbit
SIGMA/SAB4200534 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-Claudin-1 (C-Terminal), antibody produced in rabbit, affinity isolated antibody
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Claudin-1 (C-terminal) antibody produced in Rabbit (Cat. No. SAB4200534) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Claudin-1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 0.5 μg/mL.
Immunohistochemistry Localization of claudin-1 in human colon. Formalin-fixed paraffin-embedded tissue was stained with 20 μg/mL. Anti-Claudin-1 (C-terminal) (Cat. No. SAB4200534) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunofluorescence Membrane localization of claudin-1 in MDCK cells. Fixed cells were stained with 1 μg/mL Anti-Claudin-1 (C-terminal) (Cat. No. SAB4200534) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell lysates of HEK-293T overexpressing human claudin-1 (lanes 1,3) and HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-Claudin-1 (C-terminal) (Cat. No. SAB4200534) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody: 0.5 μg/mL 2. Antibody: 0.5 μg/mL 3. Antibody: 0.5 μg/mL + claudin-1 immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~23 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human colon. |
| indirect immunofluorescence: 1.0-2.0 μg/mL using MDCK cells. | |
| western blot: 0.5-1.0 μg/mL using extracts of HEK-293T cells overexpressing human claudin-1. | |
| UniProt accession no. | O95832  |
| Application: | Anti-Claudin-1 (C-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Claudin-1 directly interacts with tight junction (TJ) -associated peripheral membrane proteins zonula occludens (ZO) proteins -1/2/3. Defects in the gene encoding claudin-1 are the cause of an autosomal recessive syndrome named ichthyosis-sclerosing cholangitis neonatal (NISCH). Claudins′ expression is altered in several human cancers. Claudin-1 is frequently up-regulated in colorectal carcinomas (CRCs), resulting in tumor differentiation and progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Claudin-1, also known as claudin 1 (CLDN1), seminal metalloprotease-1 (Semp1) and ichthyosis, leukocyte vacuoles, alopecia, and sclerosing cholangitis (ILVASC), is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Claudins are characterized with four transmembrane domains and two extracellular loops, required to form tight junction strands. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminus of human claudin-1, conjugated to KLH. The corresponding sequence is highly conserved in mouse claudin-1 (single amino acid substitution) and in rat claudin-1 (90% identity). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |