Anti-Calbindin-D-28K antibody, Mouse monoclonal
SIGMA/SAB4200543 - clone CB-955, purified from hybridoma cell culture
Synonym: Monoclonal Anti-(28kD); Monoclonal Anti-D-28K; Monoclonal Anti-D-dependent calcium-binding protein; Monoclonal Anti-RTVL-H protein; Monoclonal Anti-avian-type; Monoclonal Anti-calbindin 1; Monoclonal Anti-vitamin
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Formalin fixed, paraffin-embedded, methacarn-treated sections of Rat Cerebellum were stained with 10 μg/mL Anti-Calbindin-D-28K antibody, Mouse monoclonal, Clone: CB-955 (Cat. No. SAB4200543) (A) and developed using 20 μg/mL Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B0529) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:500 Anti-Calbindin-D-28K (KD-15) antibody produced in Rabbit (Cat. No. C7354) (B). The antibody was developed using using 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Calbindin-D-28K antibodies,SAB4200543 (A) and C7354 (B) target different regions of Calbindin-D-28K show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded and antigen retrieval sections of Rat cerebellum with 10 μg/mL Mouse Anti-CALBINDIN-D-28K Clone: CB-955 (Cat. No. SAB4200543), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunoblotting MDBK cell extract was separated on SDS-PAGE and probed with Mouse Anti-CALBINDIN-D-28K Clone: CB-955 (Cat. No. SAB4200543). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CB-955, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~28 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum. |
| western blot: 2-4 μg/mL using MDBK extract. |
| Application: | • Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in: • western blotting • enzyme-linked immunosorbent assay (ELISA){31 • immunohistochemistry • immunohistochemical procedure • indirect immunofluorescence |
| Biochem/physiol Actions: | Calbindin D-28k might play a vital role as an intracellular Ca2+ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson’s disease (PD). |
| Biochem/physiol Actions: | Calbindin-D (28K) functions as a mobile or partly immobilized Ca2+ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca2+- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca2+-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K. |
| General description: | Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca2+ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells. |
| Immunogen: | purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |