Anti-Synaptophysin antibody, Mouse monoclonal
SIGMA/SAB4200544 - clone SVP-38, purified from hybridoma cell culture
Synonym: Monoclonal Anti-MRXSYP; Monoclonal Anti-SYP; Monoclonal Anti-Synaptophysin1; Monoclonal Anti-Synaptoporin; Monoclonal Anti-Syp1; Monoclonal Anti-p38
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded and antigen retrieval sections of Rat cerebellum with 10 μg/mL Mouse Anti-SYNAPTOPHYSIN Clone:SVP-38 (Cat. No. SAB4200544), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunofluorescence B35 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 10 μg/mL Mouse Anti-SYNAPTOPHYSIN Clone: SVP-38 (Cat. No. SAB4200544). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Newborn rat brain extract was separated on SDS-PAGE and probed with Mouse Anti-Synaptophysin Clone: SVP-38 (Cat. No. SAB4200544). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SVP-38, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 38 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded rat cerebellum. |
| indirect immunofluorescence: 10-20 μg/mL using B35 cells. | |
| western blot: 1-2 μg/mL using newborn rat brain extracts. |
| Application: | Anti-Synaptophysin antibody, Mouse monoclonal has been used in following immunochemical techniques: • Immunoblotting • Immunohistochemistry • Immunofluorescence |
| Biochem/physiol Actions: | Anti-Synaptophysin antibody, Mouse monoclonal recognizes rat, human, guinea-pig, and pig synaptophysin |
| Biochem/physiol Actions: | Synaptophysin (SYP) facilitates neurotransmitter release and synaptic plasticity. It is also implicated in synaptic vesicle formation and exocytosis. Mutation in the gene increases the risk of susceptibility to schizophrenia. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs). |
| Biochem/physiol Actions: | Synaptophysin has also been implicated in the recycling of synaptic vesicles by associating with dynamin I, a GTPase required for endocytosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Synaptophysin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma SVP-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synaptosome preparation from rat retina. Synaptophysin is a glycoprotein containing four transmembrane domains with both N and C termini on the cytoplasm face. |
| General description: | Synaptophysin (SYP) is encoded by the gene mapped to human chromosome Xp11.23-p11.22. The encoded protein has a molecular mass of 38kDa and is exclusively localized to the membrane of presynaptic vesicles. |
| Immunogen: | synaptosome preparation from rat retina. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |