Anti-Parvalbumin antibody, Mouse monoclonal
SIGMA/SAB4200545 - clone PARV-19, purified from hybridoma cell culture
Synonym: Monoclonal Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded and antigen retrieval sections of Rat cerebellum with 10 μg/mL Mouse Anti-PARVALBUMIN Clone: PARV-19 (Cat. No. SAB4200545), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunofluorescence BLO-11 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Mouse Anti-PARVALBUMIN Clone: PARV-19 (Cat. No. SAB4200545). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Mouse muscle extract was separated on SDS-PAGE and probed with Mouse Anti-Parvalbumin Clone: PARV-19 (Cat. No. SAB4200545). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PARV-19, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 12 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat cerebellum. |
| indirect immunofluorescence: 10-20 μg/mL using BLO-11 cells. | |
| western blot: 1-2 μg/mL using mouse muscle extracts. |
| Application: | Anti-Parvalbumin antibody, Mouse monoclonal has been used in: • immunohistochemistry • immunofluorescence staining • immunolabeling • to label brain slices for perineuronal net staining • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Ca2+ participates in cell motility, release of neurotransmitters, membrane permeability, excitation−contraction of muscles, cell secretory procedures, and transmission of nerve impulses. Intracellular Ca2+ exchangers are essential to modulate these processes efficiently. Parvalbumin (PV), a calcium-binding protein, can regulate the intracellular calcium dynamics in neurons. Hence it may have an impact on facilitation and depression at synapses. Interestingly, PV deficiency alters neuronal activity, a key mechanism leading to epileptic seizures or Parkinson. |
| Biochem/physiol Actions: | Monoclonal Anti-Parvalbumin recognizes parvalbumin in a Ca2+ -ion dependent manner |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the hybridoma PARV-19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified frog muscle parvalbumin. Parvalbumin (PV) is a member of the family of Ca2+-binding molecules. It carries three helix-loop-helix EF-hand Ca2+ binding motifs, with the two C-terminal motifs. |
| Immunogen: | purified frog muscle parvalbumin. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |