Anti-VAChT (C-terminal) antibody produced in rabbit
SIGMA/SAB4200559 - IgG fraction of antiserum
Synonym: Anti-SLC18A3; Anti-Solute carrier family 18 member 3; Anti-VAT; Anti-vesicular acetylcholine transporter
Product Type: Chemical
Immunohistochemistry Staining of frosen Rat Brain sections with 1:200 Rabbit Anti-VAChT (C-terminal) (Cat. No. SAB4200559), followed by Anti-Rabbit IgG, Cy3 conjugate. Tissue was counterstained with DAPI (blue) to stain nuclei. (The picture is courtesy of Dr. Shay Shoham, The Hebrew University of Jerusalem, Israel.)
Immunofluorescence
PC-12 cells treated with rat NGF, were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:2,000 Rabbit Anti-VAChT (C-terminal) (Cat. No. SAB4200559). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
PC-12 cells treated with rat NGF, were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:2,000 Rabbit Anti-VAChT (C-terminal) (Cat. No. SAB4200559). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 1:800 Rabbit Anti-VAChT (C-terminal) (Cat. No. SAB4200559). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Mouse Brain 2. Rat Brain 3. Rat Brain + VAChT (C-terminal) immunizing peptide 4. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:1,000-1:2,000 using PC12 cells treated with rat NGF. |
| western blot: 1:500-1:800 using extracts of mouse brain (S1 fraction). | |
| UniProt accession no. | Q16572  |
| Application: | Anti-VAChT (C-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence staining • immunocytochemistry • double immunolabeling |
| Biochem/physiol Actions: | Vesicular Acetylcholine Transporter (VAChT), is required in cholinergic neurons for the selective transport of acetylcholine (ACh) into synaptic vesicles. It depends on the activity of a vacuolar-type H+ -ATPase to accumulate ACh in synaptic vesicles. Knockdown of VAChT in the hippocampus has been shown to cause behavior changes, learning deficits, specific deficits in spatial memory and long-term potentiation (LTP). VAChT provides a specific marker for cholinergic neurons, for the study of cholinergic transmission in experimental models of Alzheimer′s disease and other nervous system disorders. Cloning of the VAChT gene has revealed a single genetic locus encoding both VAChT and ChAT, providing a unique genomic arrangement suggesting that these genes may be co-regulated. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vesicular Acetylcholine Transporter (VAChT, SLC18A3) belongs to the solute carrier family 18 (SLC18) family of proton/neurotransmitter antiporters and shares a high degree of homology to vesicular monoamine transporters (VMATs). It is a 12-transmembrane domain protein. VAChT is expressed in all major cholinergic neurons in the central and peripheral nervous system and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminus of human vesicular acetylcholine transporter (VAChT), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse VAChT (82% identity). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |