Monoclonal Anti-ZFP36/TTP antibody produced in mouse
SIGMA/SAB4200565 - clone TTP6, tissue culture supernatant
Synonym: Anti-G0/G1 switch regulatory protein 24; Anti-G0S24; Anti-Growth factor-inducible nuclear protein NUP475; Anti-NUP475; Anti-RNF162A; Anti-TIS11; Anti-TIS11A; Anti-Zinc finger protein 36 homolog; Anti-tristetraprolin; Anti-zinc finger protein 36, C3H type, homolog (mouse); Anti-zinc finger protein, C3H type, 36 homolog (mouse)
Product Type: Chemical
Immunoblotting Whole extract of HepG2 cells was separated on SDS-PAGE and probed with Mouse Anti-ZFP36/TTP Clone: TTP6 (Cat. No. SAB4200565). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:2,000 2. Antibody dilution 1:2,000 + 40 μg/mL TTP immunizing peptide (human, 51-67) 3. Antibody dilution 1:2,000 + 40 μg/mL TTP immunizing peptide (human, 270-289)
Immunoblotting Lysates were separated on SDS-PAGE and probed with Mouse Anti-ZFP36/TPP Clone: TPP6 (Cat. No. SAB4200565) diluted 1:5,000. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HepG2; 2. A431; 3. 3T3; 4. Neuro 2a; 5. PC12; 6. NRK; 7. COS-7; 8. MDBK; 9. MDCK
| antibody form | tissue culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TTP6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~45 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, bovine, rat, dog, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:4,000-1:8,000 using extracts of HepG2 cells. |
| UniProt accession no. | P26651  |
| Application: | Monoclonal Anti-ZFP36/TTP antibody has been used in immunoblotting. |
| Biochem/physiol Actions: | ZFP36 (also known as tristetraprolin or TTP) participates in controlling the expression of AU-rich element (ARE) -containing mRNAs. It participates in accelerating mRNA decay. ZFP36 was found to limit the expression of a number of critical genes frequently overexpressed in inflammation and cancer. It also plays a major role in differentiation, reprogramming, metabolism, proliferation, pluripotency, tumorigenesis and immunity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ZFP36/TTP (mouse IgM isotype) is derived from the hybridoma TTP6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal sequence of human ZFP36/TTP, conjugated keyhole limpet hemocyanin (KLH). ZFP36 (also known as tristetraprolin or TTP) is a RNA-binding protein. It belongs to the RNA-binding zinc-finger protein family. TTP consists of a cysteine-cysteine-cystein |
| Immunogen: | a peptide corresponding to an internal sequence of human ZFP36/TTP conjugated KLH. |
| Physical form: | Solution in Dulbecco′s Modified Eagle′s Medium with 10% fetal calf serum and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |