Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal
SIGMA/SAB4200571 - clone NSE-P1, purified from hybridoma cell culture
Synonym: Anti-2-phospho-D-glycerate hydrolyase; Anti-Enolase 2 (gamma, neuronal); Anti-NSE; Anti-Neural enolase; Anti-gamma-enolase; Monoclonal Anti-2-phospho-D-glycerate hydro-lyase
Product Type: Chemical
Immunocytochemistry JOURNAL CITATION: Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. By: Cheng, Y. C., Huang, C. J., et al. in Sci Rep, 2016. PubMed ID: 27444754 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/srep30314), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded sections of human cerebellum with 10 μg/mL Mouse Anti-NSE, Clone: NSE-P1 (Cat. No. SAB4200571), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded and sections of human cerebellum with 10 μg/mL Mouse Anti-NSE, Clone: NSE-P1 (Cat. No. SAB4200571), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1 μg/mL Mouse Anti-NSE Clone: NSE-P1 (Cat. No. SAB4200571). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. SH-SY5Y 2. NT2/D1 3. Neuro 2a 4. NE-4C 5. Rat brain total lysate 6. PC12
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NSE-P1, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~47 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum. |
| western blot: 0.5-1.0 μg/mL using NTERA-2 (NT2/D1) total cell extracts. | |
| UniProt accession no. | P09104  |
| Application: | Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal has been used in: • immunofluorescence staining • immunocytochemistry • immunofluorescence microscopy • immunoblotting • enzyme-linked immunosorbent assay (ELISA) • immunohistochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | Enolase 2 (ENO2) participates in glycolysis by converting β-glycerophosphate into dihydroxyacetone phosphate. It serves as a neurobiochemical marker of brain damage after traumatic brain injury, anoxic encephalopathy, stroke, and cardiac arrest. NSE also acts as a circulating marker for neuroendocrine tumors. High levels of NSE is observed in breast cancer upon exposure to arsenite and cadmium. |
| Biochem/physiol Actions: | Monoclonal Anti- Neuron-Specific Enolase (NSE) recognizes human, rat, and mouse NSE. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-neuron-specific enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Neuron specific enolase (NSE) belongs to the family of enolase enzymes. Enolases have three subunits (a, b, and g), which can combine to form five different isoenzymes: aa, ab, ag, bb, and gg. Enolase 1 (aa) is present in adipose tissue, kidney, liver, and spleen. Enolase 3 (bb) is muscle specific. Enolase 2 (ENO2), also known as neuron-specific enolase (NSE) is a cytoplasmic enzyme, that is released during cell destruction and has a half-life of approximately 30 hours in serum. This dimeric enzyme (gg) is found in neurons and in neuroendocrine cells. ENO2 gene is mapped to human chromosome 12p13. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |