Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal
SIGMA/SAB4200572 - clone NSE-P2, purified from hybridoma cell culture
Synonym: Monoclonal Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded and antigen retrieval sections of human cerebellum with 10 μg/mL Mouse Anti-NSE, Clone: NSE-P2 (Cat. No. SAB4200572), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded and antigen retrieval sections of human cerebellum with 10 μg/mL Mouse Anti-NSE, Clone: NSE-P2 (Cat. No. SAB4200572), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunoblotting Lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-NSE Clone: NSE-P2 (Cat. No. SAB4200572). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. SH-SY5Y 2. NT2/D1 3. Neuro 2a 4. NE-4C 5. Rat brain total lysate
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NSE-P2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~47 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum. |
| western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts. | |
| UniProt accession no. | P09104  |
| Application: | Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in: • immunoblotting • enzyme-linked immunosorbent assay (ELISA) • immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE. |
| Biochem/physiol Actions: | Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho- |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |