Anti-ERGIC-53 antibody, Mouse monoclonal
SIGMA/SAB4200585 - clone ERGIC-3, purified from hybridoma cell culture
Synonym: Monoclonal Anti-ER-Golgi intermediate compartment 53 kDa protein; Monoclonal Anti-ERGIC-53 antibody produced in mouse; Monoclonal Anti-ERGIC53; Monoclonal Anti-F5F8D; Monoclonal Anti-Gp58; Monoclonal Anti-Intracellular mannose-specific lectin MR60; Monoclonal Anti-LMAN1; Monoclonal Anti-Lectin mannose-binding 1
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-ERGIC-53 Clone: ERGIC-3 (Cat. No. SAB4200585). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. HepG2 3. HEK-293T 4. CHO 5. NRK 6. NIH-3T3 7. COS7 8. A-10 9. A-20
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ERGIC-3, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~58 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1-2 μg/mL using whole extracts of human HeLa cells. |
| UniProt accession no. | P49257  |
| Biochem/physiol Actions: | Endoplasmic reticulum-Golgi intermediate compartment protein 53kDa protein (ERGIC-53) plays an important role in the proliferation of arenavirus, coronavirus and filovirus. This intracellular cargo receptor enables the anterograde passage of very few glycoprotein ligands in the early exocytic pathway. Mutations in ERGIC-53 (LMAN1) gene results in the deficiency of factor V and factor VIII. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Endoplasmic reticulum-Golgi intermediate compartment 53kDa protein (ERGIC-53), a nonglycosylated, hexameric type I integral membrane protein is also known as LMAN1 (lectin, mannose-binding 1). This gene is located on human chromosome 18q21.32. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human ERGIC-53, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine and monkey. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |