Anti-WHAMM (C-terminal) Produced in rabbit
SIGMA/SAB4200590 - affinity isolated antibody
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human WHAMM was separated on SDS-PAGE and probed with Rabbit Anti-WHAMM (C-terminal) (Cat. No. SAB4200590). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody+ WHAMM immunizing peptide 4. Negative control: without 1st antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human WHAMM. |
| UniProt accession no. | Q8TF30  |
| Application: | Anti-WHAMM (C-terminal) produced in rabbit may be used in immunoblotting. |
| Biochem/physiol Actions: | Anti- WHAMM (C-terminal) recognizes human WHAMM. |
| Biochem/physiol Actions: | WASP homologue associated with actin, membranes, and microtubules (WHAMM) takes part in actin-related protein 2 & 3 (Arp2/3)-mediated actin polymerization, mediates Golgi membrane association and microtubule dynamics. It also takes part in the maintenance of Golgi structure and participates in vesicle transport between the endoplasmic reticulum and the Golgi apparatus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | WASP homologue associated with actin, membranes, and microtubules (WHAMM) is a nucleation promoting factor. It is located in the cis-Golgi and tubulo-vesicular membrane transport intermediates. WHAMM contains an N-terminal region, a coiled-coil region and a WCA region. It is located on the human chromosome at 15q25.2. |
| Immunogen: | peptide corresponding to the C-terminal region of human WHAMM , conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |