Anti-Occludin antibody from rabbit
SIGMA/SAB4200593 - affinity isolated antibody
Synonym: BLCPMG; OCLN; PPP1R115
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded sections of human colon with 10 μg/mL Rabbit Anti-Occludin (Cat. No. SAB4200593), and developed using Biotin/ExtrAvidin®-Peroxidase (Cat No. B0529).
Immunofluorescence CaCO2 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 4 μg/mL Rabbit Anti-Occludin (Cat. No. SAB4200593). The antibody was developed using Cy3™-conjugated Goat Anti-Rabbit IgG. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Lysates were separated on SDS-PAGE and probed 1μg/mL Rabbit Anti-Occludin (Cat. No. SAB4200593). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. MCF7 2. CaCO2 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| UniProt accession no. | Q16625  |
| Application: | Anti-Occludin antibody from rabbit has been used in immunocytochemistry and western blotting. |
| Biochem/physiol Actions: | Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins, plays a vital role in formation and regulation of TJ. The encoded protein facilitates the translocation of p85a to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN is a ~65 kDa protein with four transmembrane domains including, a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops, and one intracellular turn. It is specifically localized at tight junctions (TJ). |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |