Anti-APC (C-terminal) antibody produced in rabbit
SIGMA/SAB4200594 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-Adenomatous polyposis coli protein; Anti-BTPS2; Anti-DP2; Anti-DP2.5; Anti-DP3; Anti-FAP; Anti-GS
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human duodenum sections with 20 μg/mL Rabbit Anti-APC (C-terminal) (Cat. No. SAB4200594) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence Caco-2 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2 μg/mL Rabbit Anti-APC (C-terminal) (Cat. No. SAB4200594). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei. Exposure/vibrancy may have been enhanced for clarity.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 2.5 μg/mL Rabbit Anti-APC (C-terminal) (Cat. No. SAB4200594). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. WiDr 2. HT-29 3. HepG2 4. SW620 5. HEK-293 6. HeLa
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~300 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells. |
| immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum. | |
| indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells. | |
| UniProt accession no. | P25054  |
| Application: | Anti-APC (C-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Adenomatous Polyposis Coli (APC) has a key role in the proteasome-mediated degradation of β-catenin. APC mutations cause accumulation of β-catenin in the nucleus leading to activation of LEF-1 and/or TCF, and the induction of target genes such as the oncogene c-myc. It is involved in regulation of mitotic chromosome separation and stability. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Adenomatous Polyposis Coli (APC) is a tumor suppressor gene. The APC gene encodes a large multifunctional protein that has critical functions in cell migration, adhesion and proliferation. It is a shuttling nucleo-cytoplasmic protein expressed in various tissues and its expression in the colorectal epithelium contributes to its normal growth and differentiation. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human APC, conjugated to KLH. The corresponding sequence is identical in human APC isoform b and highly conserved (single amino acid substitution) in rat and mouse APC. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |