Monoclonal Anti-FMR1 antibody produced in mouse
SIGMA/SAB4200597 - clone FMR2.5, hybridoma cell culture supernatant
Synonym: FMRP; FRAXA; POF; POF1; fragile X mental retardation 1
Product Type: Chemical
Immunofluorescence HepG2 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 10 μg/mL Mouse Anti-FMR1 Clone: FMR2.5 (Cat. No. SAB4200597). The antibody was developed using Goat Anti-Mouse IgG, Cy3™-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Extract of HepG2 cells was separated on SDS-PAGE and probed with Mouse Anti-FMR1 Clone: FMR2.5 (Cat. No. SAB4200597). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. 0.5 μg/mL antibody 5. Negative Control
Immunoblotting Extract of HepG2 cells was separated on SDS-PAGE and probed with Mouse Anti-FMR1 Clone: FMR2.5 (Cat. No. SAB4200597). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + 50 μg/mL FMR1 immunizing peptide (606-623)
Flow Cytometry 1×106 HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 5 μg/test Monoclonal Anti-FMR1, Clone: FMR2.5 (Cat. No. SAB4200597) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FMR2.5, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~71 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2.0-5.0 μg/test using HeLa cells |
| immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts. | |
| immunocytochemistry: suitable | |
| immunofluorescence: 5-10 μg/mL using HepG2 cells. | |
| UniProt accession no. | Q06787  |
| Application: | Monoclonal Anti-FMR1 antibody produced in mouse may be used in: • immunoblotting • immunocytochemistry • immunofluorescence • flow cytometry |
| Biochem/physiol Actions: | Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome. |
| Biochem/physiol Actions: | Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |