Monoclonal Anti-FTH1 antibody produced in mouse
SIGMA/SAB4200598 - ~1.0 mg/mL, clone FTH24, purified immunoglobulin
Synonym: Monoclonal Anti-FHC; Monoclonal Anti-FTH; Monoclonal Anti-FTH1; Monoclonal Anti-FTHL6; Monoclonal Anti-OK/SW-cl.84; Monoclonal Anti-PIG15; Monoclonal Anti-PLIF; Monoclonal Anti-cell proliferation-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 5 μg/mL Mouse Anti-FTH1 Clone: FTH24 (Cat. No. SAB4200598). The antibody was developed using Goat Anti-Mouse IgG, Cy3™-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Extract of HEK-293T cells overexpressing human FTH1 was separated on SDS-PAGE and probed with Mouse Anti-FTH1 Clone: FTH24 (Cat. No. SAB4200598). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + 50 μg/mL FTH1 immunizing peptide (6-19) 3. 1 μg/mL antibody + 50 μg/mL FTH1 immunizing peptide (151-165)
Flow Cytometry 1×106 HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton™ X-100. Fixed cells were labeled with 20 μg Mouse Anti-FTH1 Clone: FTH24 (Cat. No. SAB4200598) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FTH24, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 10-20 μg/test using HeLa cells |
| immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa). | |
| indirect immunofluorescence: 5-10 μg/mL using HeLa cells | |
| UniProt accession no. | P02794  |
| Application: | Monoclonal Anti-FTH1 antibody produced in mouse may be used in: • immunoblotting • immunocytochemistry • flow cytometry • immunofluorescence |
| Biochem/physiol Actions: | Ferritin heavy chain catalyzes the first step in iron storage, the oxidation of Fe(II) to Fe(III). In addition to iron buffering, heavy chain ferritin also enhances de novo thymidine biosynthesis. Serum ferritin level is the most commonly used marker for determining iron status stored in the body. It is markedly influenced by factors such as inflammation, malnutrition, malignancy, and iron overload disorders. Studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases. FTH1 can also be used as a possible prognostic marker for breast cancer. |
| Biochem/physiol Actions: | Monoclonal Anti- FTH1 recognizes human FTH1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-FTH1 (mouse IgG1 isotype) is derived from the hybridoma FTH24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTH1. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin heavy chain 1 gene is mapped on human chromosome 11q13. It is mainly found in heart and pancreas. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human FTH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |