Anti-EB3 antibody produced in rabbit
SIGMA/SAB4200606 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-EBF3; Anti-EBF3-S; Anti-MAPRE3; Anti-RP/EB family; Anti-RP3; Anti-member 3; Anti-
Product Type: Chemical
Immunoblotting Lysates were separated on SDS-PAGE and probed with 0.2 μg/mL Rabbit Anti-EB3 (Cat. No. SAB4200606). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat brain 2. Mouse brain 3. Negative control
Immunoprecipitation Rabbit Anti-EB3 (Cat. No. SAB4200606) was used to immunoprecipitate EB3 from mouse brain extract. Detection antibody: Rabbit Anti-EB3 (C-Term) (Cat. No. SAB4200605) followed by Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 5 μg/mL antibody 2. Negative control: 5 μg/mL antibody without extract 3. Negative control: without antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~32 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction) |
| immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction). | |
| UniProt accession no. | Q9UPY8  |
| Application: | Anti-EB3 antibody produced in rabbit has been used in: • immunoblotting • co-immunoprecipitation • immunocytochemistry • glutathione S-transferase (GST) pull-down assay |
| Biochem/physiol Actions: | In neurons, End-binding protein-3 (EB3) has been shown to interact with scaffold protein ankyrin-G and PSD95 and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation. Knockout of EB3 inhibits myoblasts elongation and fusion into myotubes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | End-binding protein-3 (EB3), also known as microtubule-associated protein RP/EB 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). EB proteins are among the most conserved components of the microtubules cytoskeleton. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is exclusively expressed in the central nervous system and it interacts with a CNS-specific APC homolog APCL. |
| Immunogen: | Synthetic peptide corresponding to an internal sequence of human EB3, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse EB3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |