Monoclonal Anti-NG2/CSPG4 antibody produced in mouse
SIGMA/SAB4200621 - clone 2164B6, purified from hybridoma cell culture
Product Type: Chemical
Immunofluorescence A375 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-NG2/CSPG4, Clone: 2164B6 (Cat. No. SAB4200621). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Flow Cytometry 1×106 SK-Mel-28 cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 2.5 μg/test Monoclonal Anti-NG2/CSPG4, Clone: 2164B6 (Cat. No. SAB4200621) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2164B6, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2.5-5 μg/test using SK-Mel-28 cells. |
| immunofluorescence: 2.5-5 μg/mL using A375 cells. | |
| UniProt accession no. | Q6UVK1  |
| Application: | Monoclonal Anti-NG2/CSPG4 antibody produced in mouse used in flow cytometry and immunocytochemistry. |
| Biochem/physiol Actions: | Nerve/glial antigen 2 (NG2) plays a central role in linking multiple oncogenic pathways required for malignant progression. NG2 expression in radial growth phase (RGP) human melanomas enables migration, protease activation and epithelial to mesenchymal transition, which is important in primary tumors for facilitating progression from a radial to vertical growth phase phenotype. It regulates sustained, high-level activation of key survival and growth pathways, integrin-regulated focal adhesion kinase (FAK), extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and phosphoinositide 3-kinase (PI3K)/AKT pathways. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nerve/glial antigen 2 (NG2), a high molecular mass melanoma- associated antigen (HMW-MAA), is a large integral membrane, cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan. |
| Immunogen: | a recombinant extracellular portion of human NG2 proteoglycan |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |