Monoclonal Anti-CYBA antibody produced in mouse
SIGMA/SAB4200631 - clone CB-64, purified from hybridoma cell culture
Synonym: Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol. Fixed cells were stained with 5 μg/mL Monoclonal Anti-CYBA antibody produced in Mouse, Clone: CB-64 (Cat. No. SAB4200631) followed by 1:500 Anti-Mouse IgG-Cy3. Picture B: Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-CYBA, Clone: CB-64 (Cat. No. SAB4200631). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody+ CYBA Immunizing peptide 3. Negative Control: without first antibody
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Mouse Anti-CYBA, Clone: CB-64 (Cat. No. SAB4200631). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4T1 2. PC-12 3. MDCK 4. MDBK
Flow Cytometry 1×106 HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 5 μg/test Monoclonal Anti-CYBA, Clone: CB-64 (Cat. No. SAB4200631) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CB-64, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, mouse, rat, monkey, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified, unmodified |
| technique(s) | flow cytometry: 5-10 μg/test using HeLa cells. |
| immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells. | |
| immunofluorescence: 5-10 μg/mL using HeLa cells. | |
| UniProt accession no. | P13498  |
| Application: | Monoclonal Anti-CYBA antibody produced in mouse has been used in: • immunoblotting • flow cytometry • immunofluorescence |
| Biochem/physiol Actions: | In prostate cancer cells downregulation of cytochrome b245, alpha polypeptide/light chain (CYBA) was reported to inhibited cell proliferation and colony formation, through AKT and ERK1/2 signaling pathways, indicating CYBA has a role in tumor angiogenesis and tumor growth. Mutations in this gene causes an autosomal recessive forms of chronic granulomatous disease (AR220CGD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-CYBA (mouse IgM isotype) is derived from the hybridoma CB-64 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human CYBA, conjugated to keyhole limpet hemocyanin (KLH). The small subunit, p22phox subunit, also known as cytochrome b245, alpha polypeptide/light chain (CYBA) is membrane bound,which is expressed in phagocytic, endothelial and vascular smooth muscle cells. This gene is located on human chromosome16q24 |
| Immunogen: | Synthetic peptide corresponding to a sequence at the internal region of human CYBA . |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |