Monoclonal Anti-GRAMD4 antibody produced in mouse
SIGMA/SAB4200634 - clone GR4-1, purified from hybridoma cell culture
Synonym: Death-inducing protein (DIP); GRAM domain containing 4; KIAA0767
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634). The antibody was developed using Goat Anti-Mouse IgG, Cy3™-conjugate.
Immunoblotting Whole extract of Jurkat cells was separated on SDS-PAGE and probed with Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 0.5 μg/mL antibody+ GRAMD4 Immunizing peptide
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. COS-7 2. JURKAT 3. HeLa 4. MCF7
Flow Cytometry 1×106 HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 10 μg/test Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GR4-1, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2.5-5.0 μg/test using using HeLa cells. |
| immunoblotting: 0.5-1 μg/mL using using whole extracts of Jurkat cells. | |
| immunocytochemistry: suitable | |
| immunofluorescence: 2-4 μg/mL using using HeLa cells. | |
| UniProt accession no. | Q6IC98  |
| Application: | Monoclonal Anti-GRAMD4 antibody produced in mouse has been used in: • immunoblotting • immunocytochemistry • flow cytometry |
| Biochem/physiol Actions: | GRAM domain containing 4 (GRAMD4) also known as DIP (Death-inducing protein), is a novel p53- independent pro-apoptotic target of E2F Transcription Factor 1 (E2F1), which localizes to the nucleus. Upregulation of GRAMD4 by E2F1 mediates apoptosis in karyotypically normal fibroblasts and tumor cells. GRAMD4 translocation to the mitochondria is mediated by p73- induced apoptosis. It interacts with B-cell lymphoma 2 (Bcl-2), promotes BCL2 associated X, apoptosis regulator (Bax) mitochondrial relocalization and oligomerization. It induces mitochondrial membrane permeabilization mediating the release of cytochrome c and second mitochondria-derived activator of caspases (Smac). Interestingly, GRAMD4 overexpression inhibits tumor growth. Its efficacy was more pronounced over time when combined with cisplatin, leading to prolonged growth retardation. |
| Biochem/physiol Actions: | Monoclonal Anti-GRAMD4 recognizes human and monkey GRAMD4. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-GRAMD4 (mouse IgG1 isotype) is derived from the hybridoma GR4-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. GRAM domain containing 4 (GRAMD4) is localized in the nucleus and is mapped to human chromosome 22q13.31. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminal region of human GRAMD4 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |