Monoclonal Anti−phospho−RNA polymerase II CTD (pSer5) antibody produced in rat
SIGMA/SAB4200638 - clone 1H4B6, purified from hybridoma cell culture
Synonym: CCFDN; CTD (carboxy−terminal domain, RNA polymerase II, polypeptide A) phosphatase; CTD of POLR2A; FCP1; POLR2; POLRA; RNA polymerase II subunit A C-terminal domain phosphatase; RPB1; RPBh1; RPO2; RPOL2; RpIILS; TFIIF-associating CTD phosphatase 1; hRPB221; hsRPB1; phosphatase of, subunit 1; polymerase (RNA) II (DNA directed) polypeptide A 220kDa (POLR2A); serine phosphatase FCP1a; subunit 1CTDP1; transcription factor IIF-associating CTD phosphatase 1
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 8 μg/mL Monoclonal Anti-phospho-RNA polymerase II CTD (pSer5), Clone: 1H4B6 (Cat. No. SAB4200638). The antibody was developed using Mouse Anti-Rat IgG, FITC conjugate (Cat. No. F5273).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-RNA POL II CTD (pSer5), Clone: 1H4B6 (Cat. No. SAB4200638). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. NRK; 2. NIH-3T3; 3. PC-12; 4. MDBK; 5. CFB; 6. COS-7; 7. EL4; 8. MDCK; 9. HeLa
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 1H4B6, monoclonal |
| concentration | ~1 mg/mL |
| isotype | IgG2b |
| mol wt | ~250 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse, monkey, canine |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer5) |
| technique(s) | immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells. |
| immunofluorescence: 4-8 μg/mL using using HeLa cells. | |
| UniProt accession no. | P24928  |
| Application: | Monoclonal Anti−phospho−RNA polymerase II CTD (pSer5) antibody produced in rat may be used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Monoclonal Anti-phospho-RNA polymerase II CTD (pSer5) recognizes specifically phosphor-Ser5 RNAPII but not phospho-Ser2 or phospho-Ser7. |
| Biochem/physiol Actions: | The C-terminal domain (CTD) of the RNA polymerase II is essential for polymerase activity and gene regulation.. The CTD functions to help couple transcription and processing of the nascent RNA and also plays roles in transcription elongation and termination. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. CTD is being dynamically modified during transcription cycle and the Ser 5 phosphorylation in promoter region is more prominent than the 3′ region. The phosphorylation at Ser5 and Ser7 is mediated by the cyclin dependent kinase 7 of the transcription factor II human (TFIIH). The recruitment of the capping machinery is the main function of phosphor-Ser5. However, other protein interactions require the phosphor-Ser5 as well. Mutation in the domain regions of POLR2A is implicated in developmental delay and in neurodevelopmental syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-phospho-RNA polymerase II CTD (pSer5) (rat IgG2b isotype) is derived from the hybridoma 1H4B6 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with Synthetic peptide.. The C-terminal domain (CTD) RNAPII also known as POLR2A is the largest subunit of RNA polymerase II. It comprises the heptapeptide repeats with serine and threonine residues in the C-terminal. POLR2A gene is mapped to human chromosome 17p13.1. |
| Immunogen: | Synthetic peptide corresponding to pSer5 of human RNA Polymerase II CTD repeat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |