Monoclonal Anti-Brg1 antibody produced in rat
SIGMA/SAB4200641 - clone 3G4, purified from hybridoma cell culture
Synonym: Anti-ATP-dependent helicase SMARCA4; Anti-BAF190; Anti-BAF190A; Anti-BRG1; Anti-BRG1-associated factor 190A; Anti-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with10 μg/mL Monoclonal Anti-Brg1, Clone: 3G4 (Cat. No. SAB4200641). The antibody was developed using Rabbit Anti-Rat IgG, FITC conjugate.
Immunoblotting HeLa nuclear extract was separated on SDS-PAGE and probed with Monoclonal Anti-Brg1, Clone: 3G4 (Cat. No. SAB4200641). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative control: without first antibody
Flow Cytometry 1×106 HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 2.5 μg/test Monoclonal Anti-Brg1, Clone: 3G4 (Cat. No. SAB4200641) followed by Mouse Anti-Rat IgG, FITC conjugate (Cat. No. F5273). Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G4, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~185 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2.5-5 μg/test using HeLa cells |
| immunoblotting: 0.1-0.2.0 μg/mL using HeLa nuclear extract | |
| immunofluorescence: 10 μg/mL using HeLa cells |
| Application: | Monoclonal Anti-Brg1 antibody produced in rat has been used in: • immunoblotting (~185 kDa) • flow cytometry • immunofluorescence |
| Biochem/physiol Actions: | Brm-related gene-1 (Brg1) (or hBrm) protein is the central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, which is involved in transcriptional modulation of hormone-responsive promoters through binding of the complex to various nuclear receptors and its recruitment to gene-specific promoters. In addition, BRG1 has also been shown to act as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. BRG1 participates in the firing of origins and the process of replication elongation. This function of BRG1 also facilitates embryogenesis. In addition, It functions as a tumor suppressor and maintains genome stability and prevent cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Brg1 (rat IgG1 isotype) is derived from the hybridoma 3G4 produced by the fusion of mouse myeloma cells (SP2) and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human Brm-related gene-1 (Brg1) protein. Brg1 or SWI/SNF (SWItch/Sucrose non-fermentable) related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A, member 4 (SMARCA4) gene with 35 exons, is encoded by the gene mapped to human chromosome 19p13. |
| Immunogen: | a synthetic peptide corresponding to a sequence at the C-terminal region of human Brg1 protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |