Monoclonal Anti-PLEKHA7 antibody produced in mouse
SIGMA/SAB4200643 - clone PLK7, purified from hybridoma cell culture
Product Type: Chemical
Immunofluorescence Caco2 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 0.5 μg/mL Monoclonal Anti-PLEKHA7, Clone: PLK7 (Cat. No. SAB4200643). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate (A). Cells were counterstained with DAPI (blue) to stain nuclei (B).
Immunoblotting Whole extract of human HEK-293T cells overexpressing PLEKHA7 flag tagged protein. was separated on SDS-PAGE and probed with Mouse Anti-PLEKHA7, Clone: PLK7 (Cat. No. SAB4200643). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. 0.5 μg/mL antibody 5. 0.25 μg/mL antibody 6. 1 μg/mL antibody + PLEKHA7 immunizing peptide 7. 1 μg/mL antibody + non relevant peptide 8. Negative control: without first Antibody
Flow Cytometry 1×106 H1299 cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 2 μg/test Monoclonal Anti-PLEKHA7, Clone: PLK7 (Cat. No. SAB4200603) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Grey histogram: negative control without first antibody, Red histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PLK7, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2-4 μg/test using NCI-H1299 cells |
| immunoblotting: suitable | |
| immunocytochemistry: 0.25-0.5 μg/mL using human CaCo2 cells | |
| UniProt accession no. | Q6IQ23   |
| Application: | Monoclonal Anti-PLEKHA7 antibody has been used in • immunoblotting • immunocytochemistry • flow cytometry |
| Biochem/physiol Actions: | Overexpression of PLEKHA7 was reported to be common in invasive lobular carcinomas (ILCs) and hence PLEKHA7 could serve as a molecular marker to differentiate ILCs from invasive ductal carcinomas (IDCs). |
| Biochem/physiol Actions: | PLEKHA7 (Pleckstrin homology domain containing, family A member 7) functions as a cytoplasmic component of the epithelial adherens junction belt, with a subcellular localization and tissue distribution and connects E-cadherin-p120 ctn complex to the microtubule cytoskeleton. It has been reported that PLEKHA7 acts as zonula adherens (ZA) component, identifies Nezha, which further binds to microtubules (MTs) of the cytoskeleton, and forms a protein complex during cytoskeleton assembly. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PLEKHA7 (mouse IgG2a isotype) is derived from the hybridoma PLK7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human PLEKHA7, conjugated to KLH. PLEKHA7 contains WW (rsp5-domain), PH (pleckstrin homology), and CC (coiled-coil) domains. |
| General description: | PLEKHA7 (Pleckstrin homology domain containing, family A member 7) is an adherens junction protein localized at the adherens junctions in colonic epithelial cells. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminal region of human PLEKHA7 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |