Monoclonal Anti-C10orf54 antibody produced in mouse
SIGMA/SAB4200644 - clone ORF.4, purified from hybridoma cell culture
Product Type: Chemical
Flow Cytometry 1×106 Platlets cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with10 μg/test Monoclonal Anti-C10orf54, Clone: ORF.4 (Cat. No. SAB4200644) followed by Rabbit Anti-Mouse IgG, FITC conjugate.A. 10 μg/test antibody specific staining B. 10 μg/test antibody+ C10orf54 immunizing peptide
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ORF.4, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 10 μg/test using human platelets |
| immunohistochemistry: suitable |
| Application: | Phosphotyrosine antibody inhibitor has been used in flow-cytometry and immunohistochemistry. |
| Biochem/physiol Actions: | Studies suggest that C10orf54 (Chromosome 10 open reading frame 54) functions as a co-inhibitory ligand and restricts the mechanism of T cell proliferation and cytokine production. It was also suggested that C10orf 54 acts as a positive regulator as well as a substrate of MT1-MMP (trans-membrane type 1-matrix metalloproteinase) during tumor growth of collagen matrix, which regulates invasive growth. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-C10orf54 (mouse IgG1 isotype) is derived from the hybridoma OR.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide.C10orf54 (Chromosome 10 open reading frame 54) is also referred to as GI24, B7 member B7-H5, VISTA (V-domain Ig suppressor of T cell activation) and Dies1. It is a novel surface glycoprotein belonging to the B7 immunoglobulin superfamily. It consists of a single IgV domain with 3 additional cysteine residues and a cytoplasmic tail. It is expressed on mature myeloid APCs (antigen-presenting cells) including peritoneal macrophages, mature BMDC (bone marrow-derived dendritic cell), neutrophils and CD11c+ DCs, and to a lesser quantity in T cells and activated Treg cells. |
| Immunogen: | synthetic peptide corresponding to a sequence at the internal region of human C10orf54 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |