Monoclonal Anti-ALIX/PDCD6IP antibody produced in mouse
SIGMA/SAB4200646 - clone ALIX-1, purified from hybridoma cell culture
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 10 μg/mL Monoclonal Anti-ALIX/PDCD6IP, Clone: ALIX-1 (Cat. No. SAB4200646). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Whole extract of human MOLT4 cells was separated on SDS-PAGE and probed with Monoclonal Anti-ALIX/PDCD6IP, Clone: ALIX-1 (Cat. No. SAB4200646). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 5 μg/mL antibody 3. Negative control: without first antibody 4. Positive control: Rabbit Anti ALIX (Cat. No. SAB4200476)
Immunoprecipitation Monoclonal Anti-ALIX/PDCD6IP, Clone: ALIX-1 (Cat. No. SAB4200646) was used to immunoprecipitate ALIX/PDCD6IP from Whole extract of human MOLT4. Detection antibody: Rabbit Anti-ALIX (C-terminal) (Cat. No. SAB4200477), followed by Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1.10 μg/test antibody 2. 20 μg/test antibody 3. Negative control: 10 μg/test non-relevant antibody 4. Whole extract of human MOLT4 cells.
Flow Cytometry 1×106 MOLT4 cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 5 μg/test Monoclonal Anti-ALIX/PDCD6IP, Clone: ALIX-1 (Cat. No. SAB4200646) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ALIX-1, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-10 μg/test using using human MOLT4 cells |
| immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells. | |
| immunofluorescence: 10-20 μg/mL using using human HeLa cells | |
| immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells. | |
| UniProt accession no. | Q8WUM4  |
| Application: | Monoclonal Anti-ALIX/PDCD6IP antibody has been used in • immunoblotting • immunoprecipitation • immunofluorescence • flow cytometry |
| Biochem/physiol Actions: | ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP). |
| Immunogen: | peptide corresponding to an internal region of human ALIX/ PDCD6IP |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |