Monoclonal Anti-CHD1 antibody produced in rat
SIGMA/SAB4200654 - clone 2F11H5, purified from hybridoma cell culture
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 μg/mL Monoclonal Anti-CHD1, Clone: 2F11H5 (Cat. No. SAB4200654). The antibody was developed using Rabbit Anti-Rat IgG, FITC conjugate.
Flow Cytometry 1×106 HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 5 μg/test Monoclonal Anti-CHD1, Clone: 2F11H5 (Cat. No. SAB4200654) followed by Rabbit Anti-Rat IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 2F11H5, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-10 μg/test using using HeLa cells |
| immunofluorescence: 20 μg/mL using using human HeLa cells. | |
| UniProt accession no. | O14646  |
| Application: | Monoclonal Anti-CHD1 antibody produced in rat may be used in: • immunofluorescence • flow cytometry • immunoblotting • immunohistochemistry |
| Biochem/physiol Actions: | Chromodomain-helicase-DNA |
| Biochem/physiol Actions: | Monoclonal Anti-CHD1 recognizes human CHD1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-CHD1 (rat IgG2a isotype) is derived from the hybridoma 2F11H5 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide. Chromodomain-helicase-DNA |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human CHD1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |