Anti-Bovine Albumin antibody produced in rabbit
SIGMA/SAB4200656 - affinity isolated antibody
Synonym: Anti-BSA
Product Type: Chemical
Immunoblotting Serum from various animals were separated on SDS-PAGE and probed with 0.125 μg/mL Rabbit Anti-Bovine Albumin (Cat. No. SAB4200656). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Fetal bovine serum 2. Goat serum 3. Human serum 4. Rabbit serum
Immunoblotting Fetal bovine serum was separated on SDS-PAGE and probed with Rabbit Anti-Bovine Albumin (Cat. No. SAB4200656). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.25 μg/mL antibody 2. 0.125 μg/mL antibody 3. 0.06 μg/mL antibody 4. 0.03 μg/mL antibody 5. 0.03 μg/mL antibody + BSA immunizing protein 6. Negative Control: without first antibody
ELISA Rabbit Anti-Bovine Albumin (Cat. No. SAB4200656) was serially diluted for indirect ELISA using 5 μg/mL BSA for coating. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chromogenic substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.03-0.06 μg/mL |
| immunoprecipitation (IP): 2.5-5 μg | |
| indirect ELISA: 0.25-0.5 μg/mL (using 5μg/mL BSA for coating) |
| Application: | Anti-Bovine Albumin antibody produced in rabbit has been used in: • ELISA • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | Albumin functions include regulation of osmotic pressure in blood and transport of fatty acids and other lipophilic compounds. Serum albumin has been reported as a valuable biomarker in diseases such as cancer, HIV diseases that need monitoring of glycemic control. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Serum albumin, the most abundant protein (30-50 mg/mL) in plasma, is a multifunctional non-glycosylated, negatively charged protein produced in the liver. It binds a wide variety of lipophilic compounds such as steroids and lipophilic hormones. |
| Immunogen: | purified BSA from Cohn Fraction V |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |