Monoclonal Anti-GOLPH3 antibody produced in mouse
SIGMA/SAB4200660 - clone GOL3-1, purified from hybridoma cell culture
Synonym: GOPP1; GPP34; MIDAS; golgi phosphoprotein 3 (coat-protein)
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1 μg/mL Monoclonal Anti-GOLPH3, Clone: GOL3-1 (Cat. No. SAB4200660). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 10 μg/mL Monoclonal Anti-GOLPH3, Clone: GOL3-1 (Cat. No. SAB4200660). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HT-29; 2. COS-7; 3. NIH-3T3; 4. P19; 5. PC-12; 6. CHO; 7. MDBK; 8. MDCK
Immunoprecipitation Monoclonal Anti-GOLPH3, Clone: GOL3-1 (Cat. No. SAB4200660) was used to immunoprecipitate GOLPH3 from Whole extract of human HeLa cell. Detection antibody: Rabbit Anti-GOLPH3 (N-terminal) (Cat. No. SAB4200341), followed by Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2.5 μg/test antibody 2. 5 μg/test antibody 3. 10 μg/test antibody 4. Negative control: 10 μg/test antibody without exctract 5. Whole extract of human HeLa cells.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GOL3-1 |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2.5-5 μg/mL using using whole extracts of rat PC-12 cells. |
| immunofluorescence: 2.5-5 μg/mL using mouse 3T3 cells | |
| immunoprecipitation (IP): 5-10 μg using using lysates of human HeLa cells | |
| UniProt accession no. | Q9H4A6  |
| Application: | Monoclonal Anti-GOLPH3 antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | GOLPH3 (golgi phosphoprotein 3) links Golgi membrane to actin cytoskeleton by interacting with MYO18A, which is an unconventional myosin. This, in turn, is crucial for Golgi architecture and vesicle trafficking. It is an oncogene, which is susceptible to amplification in cancers, and is responsible for controlling the response to the anti-cancer drug, rapamycin. It is thought to function as a coat protein during Golgi trafficking. |
| Biochem/physiol Actions: | GOLPH3 has a role in Golgi trafficking and morphology. It interacts with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. GOLPH3 is also required for Golgi function. Overexpression of GOLPH3 is correlated with hyperactivation of mTOR signaling, in human cancer cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GOLPH3 (golgi phosphoprotein 3) is an abundant phosphatidylinositol-4-ph |
| General description: | Monoclonal Anti-GOLPH3 (mouse IgG1 isotype) is derived from the hybridoma GOL3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. GOLPH3 (Golgi phosphoprotein 3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. GOLPH3 is conserved from yeast to human. This gene was described as an oncogene that is commonly amplified in human cancer and in cancer cell lines. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminal region of human Golph3 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |