Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse
SIGMA/SAB4200661 - clone DYN2-11, purified from hybridoma cell culture
Synonym: CMT2M; CMTDI1; CMTDIB; DI-CMTB; DYN2; DYNII; LCCS5; dynamin 2; dynamin 2
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with 10 μg/mL Mouse anti-Dynamin 2 (DNM2), Clone: DYN2-11 (Cat. No. SAB4200661). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Whole cell lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-Dynamin 2 (DNM2), Clone: DYN2-11 (Cat. No. SAB4200661). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HepG2; 2. A549; 3. Neuro-2a; 4. RIN5; 5. PC-12; 6. COS7; 7. MDCK
Immunoprecipitation Mouse Anti-Dynamin 2 (DNM2), Clone: DYN2-11 (Cat. No. SAB4200661) was used to immunoprecipitate DNM2 from HeLa whole cell lysate. Detection antibody: Mouse Anti-Dynamin 2 (DNM2), Clone: DYN2-11 (Cat. No. SAB4200661), followed by Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Negative control: without lysate 2. Negative control: without antibody 3. 5 μg/test antibody 4. 10 μg/test antibody 5. 50 μg/test antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DYN2-11 |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~98 kDa |
| Quality Level | 200  |
| species reactivity | mouse, human, monkey, canine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2-4 μg/mL using using whole extract of HeLa cells. |
| immunofluorescence: 5-10 μg/mL using using HeLa cells. | |
| immunoprecipitation (IP): 5-10 μg using using whole extract of HeLa cells. | |
| UniProt accession no. | P50570  |
| Application: | Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | DNM2 (dynamin 2) is a GTPase which is required for clathrin-mediated endocytosis. It is important for clathrin-coated pit maturation and clathrin-coated vesicle formation. DNM2 participates in neuronal morphology, axonal growth, centrosome cohesion, actin- and microtubular organization. Mutations in it are associated with Charcot-Marie-Tooth disease, centronuclear myopathy ADCNM (autosomal dominant centronuclear myopathy) and lethal congenital contractures syndrome type 5 (LCCS5). It is upregulated in prostate cancer. |
| Biochem/physiol Actions: | Furthermore, DNM2 is upregulated in pancreatic cancer as it activates Ras-related C3 botulinum toxin substrate 1 (Rac1). It also promotes invasive cellular migration and lamellipod protrusion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Dynamin 2 (DNM2) (mouse IgG1 isotype) is derived from the hybridoma DYN2-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Dynamin 2 (DNM2) also known as DYN2 belongs to the dynamins family of guanosine triphosphatase (GTPase) proteins. |
| General description: | The gene DNM2 (dynamin 2) is mapped to human chromosome 19p13. It is widely expressed. The protein has a catalytic amino-terminal GTPase domain, a middle domain, a pleckstrin homology domain (PH), a GTPase effector domain (GED) and a less conserved carboxyl-terminal proline/arginine rich domain (PRD). |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminal region of human DNM2 , conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |