Anti-Copeptin antibody produced in rabbit
SIGMA/SAB4200663 - affinity isolated antibody
Synonym: CT-proAVP
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 20 μg/mL Rabbit Anti-Copeptin (Cat. No. SAB4200663). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Copeptin Recombinant Protein lysate was separated on SDS-PAGE and probed with Rabbit Anti-Copeptin (Cat. No. SAB4200663). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 0.25 μg/mL antibody 3 .0.125 μg/mL antibody 4. 0.125 μg/mL antibody+ immuinzing Copeptin peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.25-0.5 μg/mL (recommended using recombinant human Copeptin trifluoroacetate) |
| immunohistochemistry: 20 μg/mL (recommended using mouse or human kidney sections) | |
| immunoprecipitation (IP): 5-10 μg (recommended using recombinant human Copeptin trifluoroacetate (C8749).) | |
| indirect ELISA: 0.25-0.5 μg/mL (recommended using human Copeptin trifluoroacetate (C8749) for coating.) | |
| UniProt accession no. | P01185  |
| Application: | Anti-Copeptin antibody produced in rabbit has been used in various immunochemical techniques including : • immunohistochemistry • immunoblotting • immunoprecipitation • enzyme linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Copeptin concentration directly reflects AVP activation and serves as a biomarker of the AVP system. Altered copeptin levels are implicated in stroke, sudden death and cardiovascular events. It can also be used as a biomarker for a list of illnesses, such as acute myocardial infarction (AMI), vasodilatory shock, metabolic syndrome, diabetes insipidus, diabetes mellitus, sepsis, septic shock, lower respiratory tract infections, hyponatremia, acute dyspnea, autosomal dominant polycystic kidney disease (ADPKD), intracerebral hemorrhage, pulmonary arterial hypertension (PAH) and head injury. |
| Biochem/physiol Actions: | The gene AVP (arginine vasopressin) encodes preprovasopressin which is processed into vasopressin, neurophysin II and glycoprotein (copeptin). Mutations in it are associated with neurohypophyseal diabetes insipidus. It also plays a crucial role in social mannerism, such as recognition, aggression, reproduction and parenting. The levels of circulating copeptin is higher in children suffering from febrile seizures. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | AVP or pre-proAVP, also known as, vasopressin-neurophysin 2-copeptin is one of the main hormones of the axis hypothalamic-pituitary adrenal. It is split into 3 chains: Arg-vasopressin, neurophysin-2 and copeptin. Copeptin is a glycopeptide with 39 amino acids, located at the C-terminal part of the pre-proAVP. It is secreted by the posterior pituitary gland and poured into the systemic circulation. |
| General description: | The gene AVP (arginine vasopressin) is mapped to human chromosome 20. It is an antidiurectic hormone which is produced by hypothalamic neurons. |
| Immunogen: | a synthetic peptide corresponding to the C-terminal region of human Copeptin |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |