Anti-Myosin (Skeletal, Slow) antibody, Mouse monoclonal
SIGMA/SAB4200670 - clone NOQ7.5.4D, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse; CMD1S; CMH1; MPD1; MYHCB; SPMD; SPMM
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rabbit tongue sections with 2.5 μg/mL Monoclonal Anti-Myosin (skeletal, slow), Clone: NOQ7.5.4D (Cat. No. SAB4200670) using biotin/ExtrAvidin®-Peroxidase
Western Blotting Whole extract of Rat skeletal muscle cells were separated on SDS-PAGE and probed with Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in Mouse, Clone: NOQ7.5.4D (Cat. No. SAB4200670). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1.25 μg/mL antibody 2. 2.5 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NOQ7.5.4D, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate. |
| immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin®-Peroxidase staining system. |
| Application: | Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse has been used in immunobloting, immunohistology, Solid-phase radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and immunoelectronmicroscopy. |
| Biochem/physiol Actions: | Myosin are actin-dependent motors. Mutations in myosins genes are associated with myopathies, blindness and hearing loss. They are involved in muscle contraction, vision, hearing, cell movements and cell invasion. |
| Biochem/physiol Actions: | Myosin is the element responsible for energy transduction and force development in heart and skeletal muscles. Myosin is known to interact with actin in muscle and in nonmuscle cells. Slow myosin, also known as β-myosin heavy chain (β-MHC) is expressed during embryogenesis as part of the cardiac myogenic program under the control of NK-2 homeobox protein-5 (NKX-2.5), myocyte enhancer factor-2C (MEF-2C), and GATA-4/5/6. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin is a hexameric protein containing two heavy chain subunits and four light chain subunits. It is a major protein component of heart and skeletal muscles. Muscle myosin heavy chain (MHC) different isoforms are expressed depending on the physiological function of the muscle: skeletal, cardiac, smooth and non-muscle. |
| Immunogen: | Myosin, purified from myofibrils isolated from human skeletal muscle, |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |