Monoclonal Anti-CDH13 antibody produced in mouse
SIGMA/SAB4200672 - clone CAD-5, purified from hybridoma cell culture
Synonym: Anti-CDHH; Anti-Cadherin 13; Anti-P105
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 5 μg/mL Monoconal Anti-CDH13, Clone: CAD-5 (Cat. No. SAB4200672). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Monoconal Anti-CDH13, Clone: CAD-5 (Cat. No. SAB4200672). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2.5 μg/mL antibody 2. 5 μg/mL antibody 3. 5 μg/mL antibody + CDH13 immunizing protein
Immunoprecipitation Monoclonal Anti-CDH13, Clone: CAD-5 (Cat. No. SAB4200672) was used to immunoprecipitate CDH13 from HeLa whole cell lysate. Detection antibody: Monoclonal Anti-CDH13, followed by Goat Anti-mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 5 μg/test antibody 2. 10 μg/test antibody 3. Negative control: 10 μg/test non-relevant antibody 4. Total HeLa whole cell lysate
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CAD-5, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 5-10 μg/mL using whole extract of human HeLa cells. |
| immunohistochemistry: 5-10 μg/mL using Biotin/ExtrAvidin®-Peroxidase staining system of heat-retrieved formalin-fixed, paraffin-embedded human heart sections | |
| immunoprecipitation (IP): 5-10 μg/mL using whole extract of human HeLa cells. | |
| UniProt accession no. | P55290  |
| Application: | Monoclonal Anti-CDH13 antibody produced in mouse may be used in: • immunoblotting • immunoprecipitation • immunohistochemistry |
| Biochem/physiol Actions: | Cadherin (CDH) is involved in mediating Ca2+-dependent cell-cell adhesion. Abnormalities in the CDH13 gene have been identified in human malignancies. Promoter methylation of CDH13 results in gene silencing in non-small cell lung carcinoma (NSCLC). CDH13 is involved in cell migration, axon guidance and neurite growth. It is also associated with attention-deficit/hyperac |
| Biochem/physiol Actions: | Monoclonal Anti-CDH13 recognizes human Cadherin 13. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-CDH13 (mouse IgG1 isotype) is derived from the hybridoma CAD-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cadherin 13 (CDH13) is a member of the cadherins multigene family of single-chain glycoprotein receptors. It is a glycosylphosphatidylinosi |
| Immunogen: | N-terminal His-tagged recombinant protein of human Cadherin 13 |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |