Monoclonal Anti-α-Amylase 1A antibody produced in mouse
SIGMA/SAB4200673 - clone AMY-7, purified from hybridoma cell culture
Synonym: AMY1; AMY1A; alpha 1A (salivary); amylase
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human pancreas sections with 10 μg/mL Monoconal Anti-α-Amylase 1A, Clone: AMY-7 (Cat. No. SAB4200673). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extracts were separated on SDS-PAGE and probed with 5 μg/mL Monoconal Anti-α-Amylase 1A, Clone: AMY-7 (Cat. No. SAB4200673). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Mouse pancreas 2. Rat pancreas 3. Rat pancreas
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AMY-7, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~58 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.25-0.5 μg/mL using rat pancreas tissue extracts. |
| immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections. |
| Application: | Monoclonal Anti-α-Amylase 1A antibody has been used in immunoblotting, immunohistochemistry. |
| Biochem/physiol Actions: | α-Amylase 1A cleaves the glycosidic linkages in starch molecules to produce smaller saccharides such as maltotriose, maltose, and small amounts of glucose. AMY1A has been identified as an indicator in pancreas and salivary gland diseases, as well as lung cancer, ovarian cancer, plasmacytoma and thyroid adenomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- α-Amylase 1A (mouse IgG1 isotype) is derived from the hybridoma AMY-7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an N-terminal His-tagged recombinant human α. α-Amylase 1A, also known as AMY1A belongs to the α-Amylase family. AMY1A is a secreted digestive enzyme produced by the salivary glands and pancreas. |
| Immunogen: | N-terminal His-tagged recombinant human α-Amylase 1A. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |