Anti-Collagen Type I antibody, Mouse monoclonal
SIGMA/SAB4200678 - clone COL-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Collagen Type I antibody produced in mouse; OI4; alpha 1; alpha 2; collagen; type I
Product Type: Chemical
Immunohistochemistry Staining of frozen acetone fixed human tonsil sections with 7 μg/mL Monoclonal Anti collagen Type I, Clone: COL-1 (Cat. No.SAB4200678), using FITC-Anti-Mouse IgG.
Immunoblotting Recombinant Human Collagen Type I (Cat. No. C7999) was separated on SDS-PAGE and probed with Monoclonal Anti-Collagen Type I, Clone: COL-1 (Cat. No. SAB4200678). The antibody was developed using a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. Negative Control: without first antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | COL-1, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | pig, rat, human, deer, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| ELISA: suitable | |
| immunoblotting: 1-2 μg/mL using recombinant human collagen, expressed in Nicotiana tabacum | |
| immunohistochemistry: 3.5-7 μg/mL using frozen sections of human tonsil, human tongue or pig tongue |
| Application: | Monoclonal Anti-Collagen Type I antibody produced in mouse has been used in immunohistochemical analysis and in vitro invasion assays. |
| Biochem/physiol Actions: | Collagen, type I (COL1A1) is an important component of the connective tissue matrix. It plays an important role in the growth and maintenance of organ and tissue integrity. This protein also participates in the process of tissue repair. COL1A1 is implicated in fibrosis. |
| Biochem/physiol Actions: | Collagens are involved in structural roles. Additionally, they associate with various receptors and control cell proliferation, migration as well as differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Collagen type Iα1 (COL1A1) is encoded by the gene that is located on human chromosome 17q21.33. It is the most abundant extracellular matrix (ECM) protein in humans. Type 1 collagen is the major structural protein of bone, tendon, skin and cornea. The encoded protein is a heterotrimer consisting of two α1-chains and one α2-chain. |
| General description: | Collagens are present in the extracellular matrix and contains a chains which can differ in length. |
| Immunogen: | bovine skin collagen type I |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |