Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody produced in mouse
SIGMA/SAB4200679 - clone 1A4, purified from hybridoma cell culture
Synonym: AAT6; ACTSA; MYMY5; actin; alpha 2; aorta; smooth muscle
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 2 μg/mL Monoclonal Anti-Actin α-Smooth Muscle - Peroxidase, Clone:1A4 (Cat. No. SAB4200679).
Immunoblotting Whole extract of Mouse heart was separated on SDS-PAGE and probed with Monoclonal Anti-Actin α-Smooth Muscle - Peroxidase, Clone: 1A4 (Cat. No. SAB4200679). The antibody was developed using a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. Negative Control: without first antibody
Immunoblotting Whole extract of Rat heart was separated on SDS-PAGE and probed with Monoclonal Anti-Actin α-Smooth Muscle - Peroxidase, Clone: 1A4 (Cat. No. SAB4200679). The antibody was developed using a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. Negative Control: without first antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A4, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG2a |
| mol wt | antigen ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | frog, mouse, hamster, chicken, viper, bovine, crayfish, goat, rat, cat, sheep, guinea pig, lizard, human, rabbit, dog, snail |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: suitable |
| immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues | |
| immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections |
| Application: | Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in: • direct-immunoblotting • direct-immunohistochemi • direct- enzyme linked immunosorbent assay (ELISA) • direct staining of tissues and cells • characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures. |
| Biochem/physiol Actions: | Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Actin, α-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of α-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues. |
| Immunogen: | a synthetic peptide corresponding to a sequence at the NH2 terminal synthetic decapeptide of α-smooth muscle actin, conjugated to KLH. |
| Physical form: | Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline |
| Preparation Note: | Reconstitute in 0.1mL of distilled water. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |