Anti-Human IgG (Fc specific) antibody, Mouse monoclonal
SIGMA/SAB4200682 - clone GG-7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG (Fc specific), Clone: GG-7 (Cat. No. SAB4200682) was serially diluted for indirect ELISA using 5 μg/mL IgG from human serum (Cat. No. I2511) for coating. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A9917) and a chemiluminescent substrate.
ELISA Isotype specifity test: 0.3 μg/mL Monoclonal Anti-Human IgG (Fc specific), Clone: GG-7 (Cat. No. SAB4200682) was used to detect the Fc fragment of Human IgGs. Reactivity is observed with all human IgG subclasses but not with human IgA, IgM or the Fab fragment of human IgG. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A9917) and a chemiluminescent substrate.
ELISA Isotype specifity test: 0.3 μg/mL Monoclonal Anti-Human IgG (Fc specific), Clone: GG-7 (Cat. No. SAB4200682) was used to detect the Fc fragment Human IgGs. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A9917) and a chemiluminescent substrate. Columns 1. Human IgG1-Kappa 2. Human IgG1-Lambda 3. Human IgG2-Kappa 4. Human IgG2-Lambda
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GG-7, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating. |
| Application: | Monoclonal Anti-Human IgG (Fc specific) antibody has been used in: • ELISA • Imprint Immunofixation (IIF) • Immunofluorometric Assay (IFMA) • Hemagglutination (HA) • Hemagglutination Inhibition (HAI) • Particle Counting Immunoassay (PACIA) • detection of cytoplasmic IgG |
| Biochem/physiol Actions: | The crystallizable fragment (Fc) of IgG has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. IgG deficiencies are often associated with various diseases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgG (Fc specific) (mouse IgG1 isotype) is derived from the GG-7 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. |
| Immunogen: | Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |