Anti-Human IgG4 antibody, Mouse monoclonal
SIGMA/SAB4200683 - clone HP-6025, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG4 antibody produced in mouse
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG4 Clone: HP-6025 (Cat. No. SAB4200683) was serially diluted to detect Human IgG4 (lambda and kappa chain) by indirect ELISA. The antibody was developed using Goat Anti-Mouse IgG (Fab specific)-Peroxidase (Cat. No. A9917) and a chromogenic substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HP-6025, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml human IgG4 myeloma proteins for coating. |
| Application: | The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG. |
| Biochem/physiol Actions: | IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiectasia. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. IgG subclass expression pattern in malaria patients reveals positive association of IgG4 levels and malaria antigens but not with patients age. |
| Biochem/physiol Actions: | Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgG4 (mouse IgG1 isotype) is derived from the HP-6025 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG4 is a subclass of IgG with γ4 heavy chain. |
| Immunogen: | Purified human IgG4 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |