Anti-Collagen, Type VII antibody, Mouse monoclonal
SIGMA/SAB4200686 - clone LH7.2, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Collagen, Type VII antibody produced in mouse; COL7A1; EBD1; EBDCT; EBR1; alpha 1; collagen; type VII
Product Type: Chemical
Immunohistochemistry Staining of frozen acetone fixed human tonsil sections with 3 μg/mL Monoclonal Anti collagen Type VII, Clone: LH7.2 (Cat. No. SAB4200686), using FITC-Anti-Mouse IgG.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LH7.2, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: 1.5-3 μg/mL using frozen human tonsil sections |
| Application: | Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | Collagen type VII is very essential for adhesive connection between the dermis and the epidermal basement membrane zone (BMZ). Mutations in collagen type VII cause dystrophic forms of epidermolysis bullosa (including recessive dystrophic epidermolysis bullosa (RDEB)), which manifest as skin fragility and malformed enamel. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Collagen Type VII (mouse IgG1 isotype) is derived from the hybridoma LH7.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Collagen Type VII (known also as Col7), which belongs to the collagen superfamily, is a major extracellular matrix component of the anchoring fibrils in lamina densa. COL7A1 (collagen type VII alpha 1 chain) gene codes for type VII collagen. It is located on human chromosome 3p. It is expressed by keratinocytes and fibroblasts. COL7A1 is the important component the anchoring fibrils. |
| Immunogen: | insoluble fractions prepared from neonatal foreskin epidermal cells |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |