Anti-EpCAM antibody, Mouse monoclonal
SIGMA/SAB4200690 - clone Ber-EP4, hybridoma cell culture supernatant
Synonym: Anti-CD326 antigen; Anti-EGP; Anti-Epithelial cell surface antigen; Anti-Epithelial glycoprotein; Anti-KS 1/4 antigen; Anti-KSA; Anti-Major gastrointestinal tumor-associated protein GA733-2; Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human colon carcinoma sections with 1:400 Monoclonal Anti-EpCAM, Clone:Ber-EP4 (Cat. No. SAB4200690) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence MCF7 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Monoclonal Anti-EpCAM Clone:Ber-EP4 (Cat. No. SAB4200690). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Ber-EP4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:500-1:1,000 using human breast adenocarcinoma MCF-7 cell line. |
| immunohistochemistry: 1:250-1:500 using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections. | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P16422  |
| Application: | Anti-EpCAM antibody has been used in: • immunofluorescence • immunohistochemistry • immunoprecipitation |
| Biochem/physiol Actions: | EpCAM (Epithelial cell adhesion molecule) regulates cell-cell contact adhesion strength and tissue plasticity and epithelial cell proliferation and differentiation. Mutations in EpCAM are associated with several intestinal abnormalities such as Lynch syndrome, and congenital tufting enteropathy (CTE). Anti-EpCAM has been proved valuable for the distinction of undifferentiated primary or metastatic tumors from non-epithelial tumors, bile duct cells from hepatocytes in certain liver diseases, and between epithelial and normal reactive or neoplastic mesothelial cells from carcinoma cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-EpCAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma Ber-EP4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human breast carcinoma cell line MCF-7. EpCAM (Epithelial cell adhesion molecule) is a highly conserved type I transmembrane glycoprotein. EpCAM is present on most epithelia tissues of the adult body and in undifferentiated rather than differentiated embryonic stem cells. |
| Immunogen: | human breast carcinoma cell line MCF-7 |
| Physical form: | The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |