Anti-Insulin antibody, Mouse monoclonal
SIGMA/SAB4200691 - clone K36AC10, purified from hybridoma cell culture
Synonym: INS
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded A. Human, B. Mouse and C. Rat pancreas sections with 10 μg/mL Monoclonal Anti-Insulin Clone:K36AC10 (Cat. No. SAB4200691) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence b-TC-6 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-Insulin, Clone: K36AC10 (Cat. No. SAB4200691). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | K36AC10, monoclonal |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | guinea pig, porcine, bovine (proinsulin), rat, monkey, sheep, dog, horse, human (insulin ), rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: 2.5-5 μg/mL using ß -TC-6 Mouse Embryo Pancrease Insulinoma cells. | |
| immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections and Biotin/ExtrAvidin®-Peroxidase staining system. | |
| radioimmunoassay: suitable |
| Application: | Anti-Insulin antibody, Mouse monoclonal has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Insulin is responsible for two types of actions- excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Aberrant insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Mutant INS-gene Induced diabetes of youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The INS gene encodes for preproinsulin, which is enzymatically converted into insulin. Insulin is produced by the β cells in the Islets of Langerhans. Preproinsulin is converted to proinsulin in the endoplasmic reticulum and proinsulin is then proteolytically processed to form insulin in newly-forming insulin secretory granules. Insulin production is tightly regulated by specific DNA elements present within ~400 bp in the proximal region of the INS promoter. |
| Immunogen: | Human insulin |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphaste buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |