Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal
SIGMA/SAB4200692 - clone GST-2, purified from hybridoma cell culture
Synonym: GST; Glutathione-
Product Type: Chemical
Immunoblotting Recombinant GST protein from Escherichia coli (Cat. No. G5663) was separated on SDS-PAGE and probed with Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal, Clone: GST-2 (Cat. No. SAB4200692). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1.0 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.125 μg/mL antibody 5. 0.0625 μg/mL antibody 6. 0.003 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GST-2, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| ELISA: suitable | |
| immunoblotting: 0.5 μg/mL | |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable |
| Application: | Anti-Glutathione-S-Transf • immunoblotting • immunoprecipitation • dot blotting • autoradiography • immunofluorescence • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Glutathione-S-Transf |
| Immunogen: | purified recombinant GST fusion protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |