Anti-CNPase antibody, Mouse monoclonal
SIGMA/SAB4200693 - clone 11-5B, purified from hybridoma cell culture
Synonym: 2′,3′-cyclic-nucleotide 3′-phosphodiesterase; CNP; CNPase
Product Type: Chemical
Immunoblotting Whole extract of Rat brain was separated on SDS-PAGE and probed with Anti-CNPase antibody, Mouse monoclonal, Clone:11-5B (Cat. No. SAB4200693). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 10 μg/mL antibody 2. 5 μg/mL antibody 3. Negative Control: without first antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 11-5B, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 48 kDa (CNP2) |
| antigen 46 kDa (CNP1) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | pig (pig origin but not guinea pig), sheep, rat, bovine, rabbit, canine, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 5-10 μg/mL using whole rat brain extract |
| Application: | Anti-CNPase antibody, Mouse monoclonal has been used in immunoblotting, immunohistochemistry. |
| Biochem/physiol Actions: | CNPase (2α,3α-cyclic nucleotide 3α-phosphodiesterase), interacts with cytoskeletal proteins, cholesterol-rich cell membranes, nucleotides and various other proteins. Abnormalities in CNPase expression cause perturbations in myelin structure and are associated with neurological diseases such as schizophrenia and multiple sclerosis. It was shown that CNPase can bind Gag structural protein and prevent the assembly of HIV-1 particle, demonstrating the anti-viral activity of CNPase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-CNPase antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the 11-5B hybridoma produced by the fusion of mouse myeloma cells and splenocytes from RBF/Dn mouse immunized with human 2α,3α-cyclic nucleotide 3α-phosphodiesterase. CNPase (2α,3α-cyclic nucleotide 3α-phosphodiesterase), also known as CNP, is a membrane-anchored enzyme within the myelin sheath. CNPase is primarily a heterodimer of approximately 94 kDa. The heterodimer consists of a varied proportion of CNPase isoforms CNP1 (46 kDa) and CNP2 (48 kDa). |
| Immunogen: | Human 2′,3′-cyclic nucleotide 3′-phosphodiesterase |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |