Anti-Talin antibody, Mouse monoclonal
SIGMA/SAB4200694 - clone 8D4, purified from hybridoma cell culture
Synonym: Tln
Product Type: Chemical
Immunofluorescence Chicken UMNSAH/DF1 cells were fixed and permeabilized with methanol followed by aceton. Fixed cells were stained with 5 μg/mL Anti-Talin antibody, Mouse monoclonal, Clone: 8D4 (Cat. No. SAB4200694). The antibody was developed using Goat Anti-Mouse IgG Atto-488 conjugate.
Immunoblotting Whole extract of CHO cells was separated on SDS-PAGE and probed with Anti-Talin antibody, Mouse monoclonal, Clone: 8D4 (Cat. No. SAB4200694). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8D4, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Xenopus, rat, chicken, human, bovine, sheep, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunoblotting: 1-2 μg/mL using whole extract of hamster CHO cells. | |
| immunofluorescence: 2.5-5 μg/mL using chicken UMNSAH/DF1 cells. |
| Application: | Anti-Talin antibody, Mouse monoclonal has been used in: • immunoblotting • immunofluorescence • fluorescence-activated cell sorting (FACS) • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Talin is essential for integrin-mediated adhesion to the extracellular matrix (ECM). Talin links the actin cytoskeleton to integrins at the plasma membrane and presents in a variety of tissues and cell types. In cultured cells, Talin is absent in cell-cell junctions and is found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin contains multiple binding sites for the cytoskeletal protein, vinculin. It is considered as a mechanosensor, undergoing a progressive mechanoresponse to the varying forces exerted throughout adhesion and migration, such as conformational changes, disrupted or exposed ligand binding sites and exposing hidden protease cleavage sites. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Talin antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 8D4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified cytoskeletal Talin, from chicken gizzard. Talin is a high molecular weight cytoplasmic adapter protein. It is localized at a subset of adherens junctions. Talin contains an N-terminal FERM domain (the head) linked to a flexible rod comprised of 13 amphipathic helical bundles and a C-terminal helix (DD) that forms an anti-parallel dimer. It may be found either as monomer or dimer. |
| Immunogen: | purified cytoskeletal Talin, from chicken gizzard. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |