Anti-Chondroitin Sulfate antibody, Mouse monoclonal
SIGMA/SAB4200696 - clone CS-56, purified from hybridoma cell culture
Synonym: CSPG; chondroitin sulfate proteoglycan
Product Type: Chemical
Immunohistochemistry
Staining of formalin-fixed, paraffin-embedded human colon cancer sections with 2.5 μg/mL Anti-Chondroitin Sulfate antibody, Mouse monoclonal, Clone: CS-56 (Cat. No. SAB4200696) using biotin/ExtrAvidin®-Peroxidase.
Staining of formalin-fixed, paraffin-embedded human colon cancer sections with 2.5 μg/mL Anti-Chondroitin Sulfate antibody, Mouse monoclonal, Clone: CS-56 (Cat. No. SAB4200696) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence BMGE cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-Chondroitin Sulfate, Clone: CS-56 (Cat. No. SAB4200696). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified from hybridoma cell culture |
| purified immunoglobulin | |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CS-56, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgM |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, chicken, bovine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 2.5-5 μg/mL using bovine BMGE cells |
| immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon cancer sections and Biotin/ExtrAvidin ®-Peroxidase staining system. |
| Biochem/physiol Actions: | Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) stimulates the activation of integrins in dorsal root ganglion neurons. It also has a role in stimulating inflammatory cytokine expression in microglia. CSPG4 plays a vital role in brain repair. Overexpression of the gene leads to developmental delay in patients with trisomy of 15q24-qter. CSPG4 serves as a potential prognostic biomarker and therapeutic target in malignant cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-chondroitin sulfate antibody, (mouse IgM isotype) is derived from the hybridoma CS56 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with Ventral membranes of chicken gizzard fibroblasts. This antibody recognizes chondroitin sulfate containing proteoglycans (CSPG) in bovine mammary gland epithelial (BMGE) cells and also in human, chicken and mouse fibroblasts or tissues. Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) is encoded by the gene mapped to human chromosome 15q24.2-15q24.3. It is a growth-inhibitory chondroitin sulfate proteoglycan (CSPG) produced after spinal cord injury. It has a single glycosaminoglycan (GAG) chain. The protein belongs to the transmembrane chondroitin sulfate proteoglycans family. |
| Immunogen: | Ventral membranes of chicken gizzard fibroblasts |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | for continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |