Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal
SIGMA/SAB4200699 - clone TH-2, purified from hybridoma cell culture
Synonym: TH; TYH
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Tyrosine Hydroxylase, Clone: TH-2 (Cat. No. SAB4200699). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. SH-SY5Y; 2. SK-N-SH; 3. NT-2; 4. Neuro-2a; 5. PC-12; 6. COS7; 7. CHO; 8. MDBK ; 9. MDCK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TH-2, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, human, sheep, guinea pig, rat, bovine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.25-0.5 μg/mL using whole extracts of rat PC-12 cells. |
| Application: | Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal has been used in various immunochemical techniques including immunoblotting, immunohistochemistry, immunofluorescence and immunoprecipitation. |
| Biochem/physiol Actions: | Tyrosine Hydroxylase (TH) catalyzes the hydroxylation of L-tyrosine to L-3,4-dihydroxyphenylalan |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TH-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat Tyrosine Hydroxylase. Tyrosine Hydroxylase (TH) also known as tyrosine 3- monooxygenase, is encoded by the gene mapped to human chromosome 11p15.5. It is a tetrameric enzyme composed of four subunits. Four TH Isoforms were identified in humans. |
| Immunogen: | purified rat Tyrosine Hydroxylase |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |