Anti-Chicken Egg Albumin (Ovalbumin) antibody, Mouse monoclonal
SIGMA/SAB4200702 - clone OVA-14, purified from hybridoma cell culture
Synonym: Albumin; Plakalbumin; chicken egg
Product Type: Chemical
ELISA Anti-Chicken Egg Albumin (Ovalbumin) antibody, Mouse monoclonal, Clone: OVA-14 (Cat. No. SAB4200702) was serially diluted to detect Albumin from chicken egg white (Cat. No. A5503) by indirect ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | OVA-14, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 45 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, turkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | competitive inhibition ELISA: suitable |
| dot blot: suitable | |
| immunoblotting: suitable | |
| indirect ELISA: 0.1-0.2 μg/mL using 10 μg/mL Albumin from chicken egg white (A5503) for coating. |
| Biochem/physiol Actions: | Ovalbumin (OVA) peptide 323-339 mediates 25–35% of T-cell response to intact OVA in isolated BALB/c mouse. OVA 323-339 has been widely used to determine the characteristics of class II major histocompatibility complex (MHC)-peptide binding and T-cell activation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Chicken egg albumin (ovalbumin) antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma OVA-14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken egg albumin. The product reacts with turkey egg albumin, but not with serum albumin of the following species: chicken, turkey, human, bovine, pig, donkey, goat, sheep, horse, dog, cat, guinea pig, rabbit, rat, mouse, and pigeon. Ovalbumin (323-339) is the most abundant egg white protein surrounded by B-cell epitopes, which are bound by specific IgE antibodies. In addition, it also contains CD4+ T cell epitopes. Ovalbumin is a monomeric protein with a molecular mass of 45000Da. |
| Immunogen: | purified Chicken Egg Albumin |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |